May 22, 2025 Dataset Open Access

Thiele, Kristin; Arck, Petra Clara

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{"@context":"https://schema.org/","@id":"http://doi.org/10.25592/uhhfdm.17579","@type":"Dataset","creator":[{"@id":"https://orcid.org/0000-0002-9343-9834","@type":"Person","affiliation":"Division of Experimental Feto-Maternal Medicine, Department of Obstetrics and Fetal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.","name":"Thiele, Kristin"},{"@id":"https://orcid.org/0000-0002-2932-926X","@type":"Person","affiliation":"Division of Experimental Feto-Maternal Medicine, Department of Obstetrics and Fetal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.","name":"Arck, Petra Clara"}],"datePublished":"2025-05-22","description":"<p>Data set of FCS files from murine tissue&nbsp;samples (please see xlsx file for detailed sample description). They represent the raw data files for flow cytometry data published in Figure 2, Figure 3, Figure 5, Figure 7 and Figure 8&nbsp;of the publication &quot;Pregnancy-acquired memory CD4<sup>+</sup>&nbsp;regulatory T cells improve pregnancy outcome in mice&quot; by Thiele, K. et. al.&nbsp;published in Nature Communication (DOI: 10.1038/s41467-025-61572-w).</p>\n\n<p>Abstract of the publication:</p>\n\n<p>Subsequent pregnancies are generally less prone to obstetric complications. A successful pregnancy outcome requires pivotal immunological adaptation to ensure immune tolerance towards the fetus. Thus, the lower risk for pregnancy complication during subsequent pregnancies may be attributable to immune memory mounted during first pregnancies. Here we identify higher frequencies of fetal-antigen-specific CD4<sup>+</sup>&nbsp;regulatory T (Treg) cells both postpartum and in subsequent pregnancies in mice which are partly originating from trans-differentiated Th17 cells. Our functional experiments demonstrate that these CD4<sup>+</sup>&nbsp;Treg cells have memory functions (CD4<sup>+</sup>&nbsp;mTreg) and account for an improved fetal development and pregnancy outcome, also during adverse conditions, such as gestational sound stress. Using a high-throughput single-cell quantification method, we identify candidate markers for the detection of CD4<sup>+</sup>&nbsp;mTreg cells, which include CXCR4 and CD274. Our findings thus contribute to the improved understanding of pregnancy-induced immune memory and foster the identification of immune targets aiming to reduce the risk for immune-mediated pregnancy complications.</p>","distribution":[{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-10_020.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-1_010.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-2_019.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-3_020.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-4_021.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-5_010.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-6_011.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-7_007.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-8_007.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-1-9_019.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-10_007.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-1_011.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-2_022.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-3_023.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-4_013.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-5_014.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-6_015.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-7_021.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-8_010.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_661-2-9_011.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-10_008.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-11_015.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-12_016.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-13_008.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-14_011.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-4_014.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-5_014.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-6_015.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-7_016.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.de/api/files/633ed123-e70c-481d-8fc0-ba1bf94f3a83/Blood_672-1-8_008.fcs","encodingFormat":"fcs"},{"@type":"DataDownload","contentUrl":"https://www.fdr.uni-hamburg.d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